Title

Generating Stable Bioluminescent Cell Lines by Retroviral Transduction

Authors

Jeffrey Hayashi
Andrew Gunderson
Tomoko Yamazaki
Kayla McCarty
Alejandro Alice
Michael Gough
Kristina Young

Date

Fall 2018

Faculty Advisor

Kristina Young

Sponsor

Providence Cancer Center, Earle A. Chiles Research Institute, The Oregon Clinic

College/School

College of Arts & Sciences

Department

Department of Biology

Abstract

This project focuses on improving tumor in vivo imaging techniques. It is difficult to accurately in vivo image orthotopic tumors using CT scans alone. Muriglo provides the capability to visualize in vivo bioluminescent signals emanating from intact tissues. We can coregister CT scans captured with the SARRP with luciferase signals captured with the Muriglo and locate tumor isocenters for image-guided radiation. We may then direct radiation to neoplastic tissue while also sparing the maximum amount of healthy tissue. To do this we must genetically engineer cells to express luciferase. We propose a protocol to use a retrovirus to create stable cell lines that express firefly luciferase for bioluminescence. We will also determine the optimal dosage of the G418 antibiotic for selection.

Subjects

Biology--Research; Tumors; Oncology; Bioluminescence

Publication Information

A Summer Research Celebration Project

© 2018 The Authors

Citation: Pilot Scholars Version (Modified MLA Style)

Hayashi, Jeffrey; Gunderson, Andrew; Yamazaki, Tomoko; McCarty, Kayla; Alice, Alejandro; Gough, Michael; and Young, Kristina, "Generating Stable Bioluminescent Cell Lines by Retroviral Transduction" (2018). Biology Undergraduate Publications, Presentations and Projects. 11.
https://pilotscholars.up.edu/bio_studpubs/11

Document Type

Student Project