Publication Date
11-1996
Abstract
During gastrulation of Xenopus embryos the prospective mesoderm is induced initially with domains of dorsal and ventral fate, then further patterned to generate somitic mesoderm by signals from the gastrula organizer. Although Xwnt-8 is expressed in future ventrolateral mesoderm and induces prospective epidermis to differentiate in vitro as ventral mesoderm, no loss-of-function studies have demonstrated a requirement for Wnt signaling for the normal expression of mesodermal genes in the gastrula. We report development of a dominant-negative Wnt (dnXwnt-8) that inhibits embryonic responses to Wnt signaling in a cell-nonautonomous fashion. By expressing dnXwnt-8 in Xenopus embryos, we uncover a requirement of Wnt signaling for localized expression in prospective mesoderm of XMyoDa and Xenopus-posterior (Xpo). Because ectopic expression of functional Xwnt-8 in the dorsal marginal zone of the gastrula induces ectopic XMyoDa and Xpo, both gain-of-function and loss-of-function experiments support a model in which endogenous Xwnt-8 functions to induce expression of genes involved in specification of ventral and somitic mesoderm.
Author Supplied Keywords
Mesoderm, muscel, MyoD, Wnt, xenopus
Subjects
Cytology; Molecular biology--Research
Citation: Pilot Scholars Version (Modified MLA Style)
Hoppler, Stephan; Brown, Jeffrey D.; and Moon, Randall T., "Expression of a dominant negative Wnt blocks induction of MyoD in Xenopus embryos" (1996). Biology Faculty Publications and Presentations. 4.
https://pilotscholars.up.edu/bio_facpubs/4
DOI
10.1101/gad.10.21.2805
Peer-Reviewed
Yes
Document Type
Journal Article
Publication Information
Genes & Development, 1996, Volume 10, Issue 21, 2805-2817.
© Cold Spring Harbor Laboratory Press
Archived version is the final published version.